Dimerisation of the Yeast K⁺ Translocation Protein Trk1 Depends on the K⁺ Concentration.

ABSTRACT: In baker's yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K⁺ uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel α subunit. Because most K-channels are proteins containing four channel-building α subunits, Trk1 could be functional as a monomer. However, related SKT proteins TrkH and KtrB were crystallised as dimers, and for Trk1, a tetrameric arrangement has been proposed based on molecular modelling. Here, based on Bimolecular Fluorescence Complementation experiments and single-molecule fluorescence microscopy combined with molecular modelling; we provide evidence that Trk1 can exist in the yeast

SUBMITTER: Kulik N 

PROVIDER: S-EPMC9820094 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature