¹²⁹Xe NMR-Protein Sensor Reveals Cellular Ribose Concentration.

ABSTRACT: Dysregulation of cellular ribose uptake can be indicative of metabolic abnormalities or tumorigenesis. However, analytical methods are currently limited for quantifying ribose concentration in complex biological samples. Here, we utilize the highly specific recognition of ribose by ribose-binding protein (RBP) to develop a single-protein ribose sensor detectable via a sensitive NMR technique known as hyperpolarized ¹²⁹Xe chemical exchange saturation transfer (hyper-CEST). We demonstrate that RBP, with a tunable ribose-binding site and further engineered to bind xenon, enables the quantitation of ribose over a wide concentration range (nM to mM). Ribose binding induces the RBP "closed" conformation, which slows Xe exchange to a rate detectable by hyper-CEST. Such detection

SUBMITTER: Zemerov SD 

PROVIDER: S-EPMC7649717 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature