Detecting protein-protein interactions by Xe-129 NMR.
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ABSTRACT:
SUBMITTER: Zhao Z
PROVIDER: S-EPMC7511426 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Publications
Detecting protein-protein interactions by Xe-129 NMR.
Zhao Zhuangyu Z Roose Benjamin W BW Zemerov Serge D SD Stringer Madison A MA Dmochowski Ivan J IJ
Chemical communications (Cambridge, England) 20200901 75
Detection of protein-protein interactions (PPIs) is limited by current bioanalytical methods. A protein complementation assay (PCA), split TEM-1 β-lactamase, interacts with xenon at the interface of the TEM-1 fragments. Reconstitution of TEM-1-promoted here by cFos/cJun leucine zipper interaction-gives rise to sensitive 129Xe NMR signal in bacterial cells. ...[more]
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