Detecting protein-protein interactions by Xe-129 NMR.

ABSTRACT: Detection of protein-protein interactions (PPIs) is limited by current bioanalytical methods. A protein complementation assay (PCA), split TEM-1 β-lactamase, interacts with xenon at the interface of the TEM-1 fragments. Reconstitution of TEM-1-promoted here by cFos/cJun leucine zipper interaction-gives rise to sensitive 129Xe NMR signal in bacterial cells.

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC7511426 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature