Termination of CD40L co-stimulation promotes human B cell differentiation into antibody-secreting cells.

ABSTRACT: Human naïve B cells are notoriously difficult to differentiate into antibody-secreting cells (ASCs) in vitro while maintaining sufficient cell numbers to evaluate the differentiation process. B cells require T follicular helper (T_FH ) cell-derived signals like CD40L and IL-21 during germinal center (GC) responses to undergo differentiation into ASCs. Cognate interactions between B and T_FH cells are transient; after T_FH contact, B cells cycle between GC light and dark zones where T_FH contact is present and absent, respectively. Here, we elucidated that the efficacy of naïve B cells in ACS differentiation is dramatically enhanced by the release of CD40L stimulation. Multiparameter phospho-flow and transcription factor (TF)-flow cytometry revealed t

SUBMITTER: Marsman C 

PROVIDER: S-EPMC9825913 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature