InvitroSPI and a large database of proteasome-generated spliced and non-spliced peptides.

ABSTRACT: Noncanonical epitopes presented by Human Leucocyte Antigen class I (HLA-I) complexes to CD8⁺ T cells attracted the spotlight in the research of novel immunotherapies against cancer, infection and autoimmunity. Proteasomes, which are the main producers of HLA-I-bound antigenic peptides, can catalyze both peptide hydrolysis and peptide splicing. The prediction of proteasome-generated spliced peptides is an objective that still requires a reliable (and large) database of non-spliced and spliced peptides produced by these proteases. Here, we present an extended database of proteasome-generated spliced and non-spliced peptides, which was obtained by analyzing in vitro digestions of 80 unique synthetic polypeptide substrates, measured by different mass spectrometers. Peptides were ide

SUBMITTER: Roetschke HP 

PROVIDER: S-EPMC9832164 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature