A systematic exploration of ginsenoside Rg5 reveals anti-inflammatory functions in airway mucosa cells.

ABSTRACT:

Background

Hyperactivated airway mucosa cells overproduce mucin and cause severe breathing complications. Here, we aimed to identify the effects of saponins derived from Panax ginseng on inflammation and mucin overproduction.

Methods

NCI-H292 cells were pre-incubated with 16 saponins derived from P. ginseng, and mucin overproduction was induced by treatment with phorbol 12-myristate 13-acetate (PMA). Mucin protein MUC5AC was quantified by enzyme-linked immunosorbent assay, and mRNA levels were analyzed using quantitative polymerase chain reaction (qPCR). Moreover, we performed a transcriptome analysis of PMA-treated NCI-H292 cells in the absence or presence of Rg5, and differential gene expression was confirmed using qPCR. Phosphorylation levels of signaling mo

SUBMITTER: Heo H 

PROVIDER: S-EPMC9834007 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature