The effect of repeat length on Marcal1-dependent single-strand annealing in Drosophila.

ABSTRACT: Proper repair of DNA double-strand breaks is essential to the maintenance of genomic stability and avoidance of genetic disease. Organisms have many ways of repairing double-strand breaks, including the use of homologous sequences through homology-directed repair. While homology-directed repair is often error free, in single-strand annealing homologous repeats flanking a double-strand break are annealed to one another, leading to the deletion of one repeat and the intervening sequences. Studies in yeast have shown a relationship between the length of the repeat and single-strand annealing efficacy. We sought to determine the effects of homology length on single-strand annealing in Drosophila, as Drosophila uses a different annealing enzyme (Marcal1) than yeast. Using an in vivo single-stra

SUBMITTER: Dewey EB 

PROVIDER: S-EPMC9836020 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature