Generation of myocyte agonal Ca²⁺ waves and contraction bands in perfused rat hearts following irreversible membrane permeabilisation.

ABSTRACT: Although irreversible cardiomyocyte injury provokes intracellular Ca²⁺ ([Ca²⁺]_i) overload, the underlying dynamics of this response and its effects on cellular morphology remain unknown. We therefore visualised rapid-scanning confocal fluo4-[Ca²⁺]_i dynamics and morphology of cardiomyocytes in Langendorff-perfused rat hearts following saponin-membrane permeabilisation. Our data demonstrate that 0.4% saponin-treated myocytes immediately exhibited high-frequency Ca²⁺ waves (131.3 waves/min/cell) with asynchronous, oscillatory contractions having a mean propagation velocity of 117.8 μm/s. These waves slowly decreased in frequency, developed a prolonged decay phase, and disappeared in 10 min resulting in high-static, fluo4-fluore

SUBMITTER: Morishita Y 

PROVIDER: S-EPMC9842683 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature