Deep Metagenomic Sequencing for Endophthalmitis Pathogen Detection Using a Nanopore Platform.

ABSTRACT:

Purpose

To evaluate the utility of nanopore sequencing for identifying potential causative pathogens in endophthalmitis, comparing culture results against full-length 16S rRNA nanopore sequencing (16S Nanopore), whole genome nanopore sequencing (Nanopore WGS), and Illumina (Illumina WGS).

Design

Cross-sectional diagnostic comparison.

Methods

Patients with clinically suspected endophthalmitis underwent intraocular vitreous biopsy as per standard care. Clinical samples were cultured by conventional methods, together with full-length 16S rRNA and WGS using nanopore and Illumina sequencing platforms.

Results

Of 23 patients (median age 68.5 years [range 47-88]; 14 males [61%]), 18 cases were culture-positive. Nanopore sequencing identified the same cultured organism

SUBMITTER: Low L 

PROVIDER: S-EPMC9850836 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature