Substrate Specificity of the Flavoenzyme BhaC₁ That Converts a C-Terminal Trp to a Hydroxyquinone.

ABSTRACT: The preparation of protein-protein, protein-peptide, and protein-small molecule conjugates is important for a variety of applications, such as vaccine production, immunotherapies, preparation of antibody-drug conjugates, and targeted delivery of therapeutics. To achieve site-selective conjugation, selective chemical or enzymatic functionalization of proteins is required. We have recently reported biosynthetic pathways in which small, catalytic scaffold peptides are utilized for the generation of amino acid-derived natural products called pearlins. In these systems, peptide amino-acyl tRNA ligases (PEARLs) append amino acids to the C-terminus of a scaffold peptide, and tailoring enzymes encoded in the biosynthetic gene clusters modify the PEARL-appended amino acid to generate a variety of n

SUBMITTER: Daniels PN 

PROVIDER: S-EPMC9850906 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature