Native Mass Spectrometry of BRD4 Bromodomains Linked to a Long Disordered Region.

ABSTRACT: The contribution of disordered regions to protein function and structure is a relatively new field of study and of particular significance as their function has been implicated in some human diseases. Our objective was to analyze various deletion mutants of the bromodomain-containing protein 4 (BRD4) using native mass spectrometry to characterize the gas-phase behavior of the disordered region connected to the folded domain. A protein with a single bromodomain but no long disordered linker displayed a narrow charge distribution at low charge states, suggesting a compact structure. In contrast, proteins containing one or two bromodomains connected to a long disordered region exhibited multimodal charge distributions, suggesting the presence of compact and elongated conformers. In the presen

SUBMITTER: Azegami N 

PROVIDER: S-EPMC9853951 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature