Microparticles as Viral RNA Carriers from Stool for Stable and Sensitive Surveillance.

ABSTRACT: Since its discovery, polymerase chain reaction (PCR) has emerged as an important technology for the diagnosis and identification of infectious diseases. It is a highly sensitive and reliable nucleic acids (NA) detection tool for various sample types. However, stool, which carries the most abundant micro-organisms and physiological byproducts, remains to be the trickiest clinical specimen for molecular detection of pathogens. Herein, we demonstrate the novel application of hydrogel microparticles as carriers of viral RNA from stool samples without prior RNA purification for real-time polymerase chain reaction (qPCR). In each microparticle of primer-incorporated network (PIN) as a self-sufficient reaction compartment, immobilized reverse transcription (RT) primers capture the viral RNA by hy

SUBMITTER: Kifaro EG 

PROVIDER: S-EPMC9857651 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature