VpdC is a ubiquitin-activated phospholipase effector that regulates Legionella vacuole expansion during infection.

ABSTRACT: Intravacuolar pathogens need to gradually expand their surrounding vacuole to accommodate the growing number of bacterial offspring during intracellular replication. Here we found that Legionella pneumophila controls vacuole expansion by fine-tuning the generation of lysophospholipids within the vacuolar membrane. Upon allosteric activation by binding to host ubiquitin, the type IVB (Dot/Icm) effector VpdC converts phospholipids into lysophospholipids which, at moderate concentrations, are known to promote membrane fusion but block it at elevated levels by generating excessive positive membrane curvature. Consequently, L. pneumophila overproducing VpdC were prevented from adequately expanding their surrounding membrane, trapping the replicating bacteria within spatially confi

SUBMITTER: Li X 

PROVIDER: S-EPMC9860323 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature