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FUSTA: leveraging FUSE for manipulation of multiFASTA files at scale.


ABSTRACT:

Motivation

FASTA files are the de facto standard for sharing, manipulating and storing biological sequences, while concatenated in multiFASTA they tend to be unwieldy for two main reasons: (i) they can become big enough that their manipulation with standard text-editing tools is unpractical, either due to slowness or memory consumption; (ii) by mixing metadata (headers) and data (sequences), bulk operations using standard text streaming tools (such as sed or awk) are impossible without including a parsing step, which may be error-prone and introduce friction in the development process.

Results

Here, we present FUSTA (FUse for faSTA), a software utility which makes use of the FUSE technology to expose a multiFASTA file as a hierarchy of virtual files, letting users operate directly on the sequences as independent virtual files through classical file manipulation methods.

Availability and implementation

FUSTA is freely available under the CeCILL-C (LGPLv3-compatible) license at https://github.com/delehef/fusta.

Supplementary information

Supplementary data are available at Bioinformatics Advances online.

SUBMITTER: Delehelle F 

PROVIDER: S-EPMC9875552 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

FUSTA: leveraging FUSE for manipulation of multiFASTA files at scale.

Delehelle Franklin F   Roest Crollius Hugues H  

Bioinformatics advances 20221129 1


<h4>Motivation</h4>FASTA files are the <i>de facto</i> standard for sharing, manipulating and storing biological sequences, while concatenated in multiFASTA they tend to be unwieldy for two main reasons: (i) they can become big enough that their manipulation with standard text-editing tools is unpractical, either due to slowness or memory consumption; (ii) by mixing metadata (headers) and data (sequences), bulk operations using standard text streaming tools (such as sed or awk) are impossible wi  ...[more]

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2022-11-11 | GSE201005 | GEO