Transcriptionally Distinct B Cells Infiltrate Allografts After Kidney Transplantation.

ABSTRACT:

Background

Following allogeneic kidney transplantation, a substantial proportion of graft loss is attributed to the formation of donor-specific antibodies and antibody-mediated rejection. B cells infiltrate kidney grafts during antibody-mediated rejection; however, the origins, repertoires, and functions of these intrarenal B cells remain elusive.

Methods

Here, we use murine allogeneic kidney transplant models to study the origins, transcriptional programming and B cell receptor repertoire of intragraft B cells, and in vitro stimulation assays to evaluate the ability of intragraft B cells to promote CD4+ T cell expansion.

Results

B cells infiltrate kidney grafts in settings of allogeneic, but not syngeneic, transplantation. Intragraft B cells have characteristics of a

SUBMITTER: Zhang H 

PROVIDER: S-EPMC9877106 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature