Differential Expression of Inflammarafts in Macrophage Foam Cells and in Nonfoamy Macrophages in Atherosclerotic Lesions-Brief Report.

ABSTRACT:

Background

Reprogramming of monocytes and macrophage manifests in hyperinflammatory responses and chronification of inflammation in atherosclerosis. Recent studies focused on epigenetic, transcriptional, and metabolic alterations that characterize trained immunity. However, the underlying effector mechanisms driving the hyperinflammatory response of reprogrammed macrophages remain unclear. We hypothesized that the plasma membrane of atherosclerotic lesion macrophages undergoes reprogramming to maintain inflammarafts, enlarged lipid rafts (LR) serving as a platform for assembly of inflammatory receptor complexes.

Methods

Single-cell suspensions from the aortae of Western diet-fed Ldlr^-/- mice were gated for BODIPY-high foamy and BODIPY-low nonfoamy F4

SUBMITTER: Navia-Pelaez JM 

PROVIDER: S-EPMC9877149 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature