A New Autosomal Myh11-CreER^T2 Smooth Muscle Cell Lineage Tracing and Gene Knockout Mouse Model-Brief Report.

ABSTRACT:

Background

The Myh11 promoter is extensively used as a smooth muscle cell (SMC) Cre-driver and is regarded as the most restrictive and specific promoter available to study SMCs. Unfortunately, in the existing Myh11-CreER^T2 mouse, the transgene was inserted on the Y chromosome precluding the study of female mice. Given the importance of including sex as a biological variable and that numerous SMC-based diseases have a sex-dependent bias, the field has been tremendously limited by the lack of a model to study both sexes. Here, we describe a new autosomal Myh11-CreER^T2 mouse (referred to as Myh11-CreER^T2-RAD), which allows for SMC-specific lineage tracing and gene knockout studies in vivo using both male and female mice.

SUBMITTER: Deaton RA 

PROVIDER: S-EPMC9877184 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature