Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium.

ABSTRACT: Retinal pigment epithelium (RPE) has essential functions, such as nourishing and supporting the neural retina, and is of vital importance in the pathogenesis of age-related retinal degeneration. However, the exact molecular changes of RPE during aging remain poorly understood. Here, we isolated human primary RPE (hRPE) cells from 18 eye donors distributed over a wide age range (10-67 years old). A quantitative proteomic analysis was performed to analyze changes in their intracellular and secreted proteins. Age-group related subtypes and age-associated proteins were revealed and potential age-associated mechanisms were validated in ARPE-19 and hRPE cells. The results of proteomic data analysis and verifications suggest that RNF123- and RNF149-related protein ubiquitination plays an importan

SUBMITTER: Jin X 

PROVIDER: S-EPMC9880895 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature