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Identification of age-associated proteins and functional alterations in human primary retinal pigment epithelium cells

ABSTRACT: Retinal pigmented epithelium (RPE) has essential functions to nourish and support the neural retina, and is of vital importance in the pathogenesis of age-related retinal degeneration. However, the exact molecular changes of RPE in aging remain poorly defined. We isolated human primary RPE (hRPE) cells from 18 eye donors distributed over a wide age range (10 - 67 years). A quantitative proteomic analysis was performed to analyze their intracellular and secreted protein changes. Age-stage related subtypes and age-associtated proteins were revealed and potential age-associtated mechanisms were validated by ARPE-19 and hRPE cells. Proteomic data and verifications showed that RNF123 and RNF149 related ubiquitin-mediated proteolysis might be an important clearance mechanism in elimination of oxidative damaged proteins in aged hRPE. In older hRPE cells, apoptotic signaling related pathways were up-regulated and endoplasmic reticulum organization was down-regulated both in intracellular and secreted proteome. Our work paints a detailed molecular picture of human RPE in aging process and provides new insights for molecular characteristics of RPE in aging and related clinical retinal conditions.

ORGANISM(S): Homo Sapiens

SUBMITTER: Bo Lei

PROVIDER: PXD028202 | iProX | Thu Sep 02 00:00:00 BST 2021

REPOSITORIES: iProX

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