SEC-TMT facilitates quantitative differential analysis of protein interaction networks.

ABSTRACT: The majority of cellular proteins interact with at least one partner or assemble into molecular-complexes to exert their function. This network of protein-protein interactions (PPIs) and the composition of macromolecular machines differ between cell types and physiological conditions. Therefore, characterizing PPI networks and their dynamic changes is vital for discovering novel biological functions and underlying mechanisms of cellular processes. However, producing an in-depth, global snapshot of PPIs from a given specimen requires measuring tens to thousands of LC-MS/MS runs. Consequently, while recent works made seminal contributions by mapping PPIs at great depth, almost all focused on just 1-2 conditions, generating comprehensive but mostly static PPI networks. In this study we report

SUBMITTER: Doron-Mandel E 

PROVIDER: S-EPMC9882152 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature