Comparison between qPCR and RNA-seq reveals challenges of quantifying HLA expression.

ABSTRACT: Human leukocyte antigen (HLA) class I and II loci are essential elements of innate and acquired immunity. Their functions include antigen presentation to T cells leading to cellular and humoral immune responses, and modulation of NK cells. Their exceptional influence on disease outcome has now been made clear by genome-wide association studies. The exons encoding the peptide-binding groove have been the main focus for determining HLA effects on disease susceptibility/pathogenesis. However, HLA expression levels have also been implicated in disease outcome, adding another dimension to the extreme diversity of HLA that impacts variability in immune responses across individuals. To estimate HLA expression, immunogenetic studies traditionally rely on quantitative PCR (qPCR). Adoption of altern

SUBMITTER: Aguiar VRC 

PROVIDER: S-EPMC9883133 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature