In vitro gut microbiome response to carbohydrate supplementation is acutely affected by a sudden change in diet.

ABSTRACT:

Background

Interactions between diet, stress and the gut microbiome are of interest as a means to modulate health and performance. Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota. Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions. 16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential. Specific statistical analyses were implemented to detect

SUBMITTER: Pantoja-Feliciano IG 

PROVIDER: S-EPMC9883884 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature