Lappaol F regulates the cell cycle by activating CDKN1C/p57 in human colorectal cancer cells.

ABSTRACT:

Context

Lappaol F (LAF), a natural lignan from Arctium lappa Linné (Asteraceae), inhibits tumor cell growth in vitro and in vivo. The underlying mechanism involves the suppression of the Yes-associated protein. However, the specific role of LAF in cell cycle regulation remains unknown.

Objective

This study determined the molecular mechanism by which LAF regulates cell cycle progression.

Materials and methods

Various colon cancer cell lines (SW480, HCT15, and HCT116) were treated with LAF (25, 50, and 75 μmol/L) for 48 h. The effects of LAF on cell proliferation and cell cycle were determined using sulforhodamine B and flow cytometry assays. Differentially expressed proteins (DEPs) were identified using quantitative proteomics. Bioinformatic analys

SUBMITTER: Yang RY 

PROVIDER: S-EPMC9888477 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature