Dataset Information

Evaluation of serum miR-216a and miR-375 as biomarkers in dogs with acute pancreatitis.

ABSTRACT:

Background

Serum microRNAs have emerged as biomarkers of various diseases. Overexpression of serum miR-216a and miR-375 occurs in dogs with experimentally induced acute pancreatitis (AP).

Objectives

To identify the possibility of using serum miR-216a and miR-375 as biomarkers for the diagnosis and evaluation of treatment response in dogs with naturally occurring AP.

Animals

Twenty-one dogs with AP and 20 healthy dogs.

Methods

Cross-sectional study. The relative expression of serum hsa-miR-216a-5p, cfa-miR-216a, and cfa-miR-375 were analyzed using reverse transcription and real-time PCR.

Results

A significant difference in the serum expression of cfa-miR-375 was found between dogs with AP (median [interquartile range] 3.59 [1.55-24.52]-fold) and healthy dogs (0.81 [0.54-2.21]-fold, P < .001), and no significant differences were observed in hsa-miR-216a-5p and cfa-miR-216a (P > .05). The area under the receiver operating characteristic curve of serum cfa-miR-375 for differentiating between AP dogs and healthy dogs was 0.84 (95% confidence interval [CI]: 0.71-0.96). The expressions of hsa-miR-216a-5p and cfa-miR-375 were positively correlated with the concentrations of serum C-reactive protein (r_s = .46, r_s = .48, respectively), but not with the serum specific canine pancreatic lipase. The expression of cfa-miR-375 was significantly less after treatment in dogs with AP (P = .02).

Conclusions and clinical importance

Serum cfa-miR-375 could be a potential biomarker for the diagnosis and evaluation of treatment response of AP in dogs. In addition, miR-216a and miR-375 could be associated with inflammatory processes in dogs with AP.

SUBMITTER: Lee D

PROVIDER: S-EPMC9889605 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Publications

Evaluation of serum miR-216a and miR-375 as biomarkers in dogs with acute pancreatitis.

Lee Dohee D Yun Taesik T Koo Yoonhoi Y Chae Yeon Y Choi Minseok M Kang Byeong-Teck BT Yang Mhan-Pyo MP Kim Hakhyun H

Journal of veterinary internal medicine 20221203 1

<h4>Background</h4>Serum microRNAs have emerged as biomarkers of various diseases. Overexpression of serum miR-216a and miR-375 occurs in dogs with experimentally induced acute pancreatitis (AP).<h4>Objectives</h4>To identify the possibility of using serum miR-216a and miR-375 as biomarkers for the diagnosis and evaluation of treatment response in dogs with naturally occurring AP.<h4>Animals</h4>Twenty-one dogs with AP and 20 healthy dogs.<h4>Methods</h4>Cross-sectional study. The relative expre ...[more]

PMID: 36461714

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Dataset Information

Evaluation of serum miR-216a and miR-375 as biomarkers in dogs with acute pancreatitis.

Background

Objectives

Animals

Methods

Results

Conclusions and clinical importance

Publications

Evaluation of serum miR-216a and miR-375 as biomarkers in dogs with acute pancreatitis.

Similar Datasets

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