Evaluation of serum miR-216a and miR-375 as biomarkers in dogs with acute pancreatitis.

ABSTRACT:

Background

Serum microRNAs have emerged as biomarkers of various diseases. Overexpression of serum miR-216a and miR-375 occurs in dogs with experimentally induced acute pancreatitis (AP).

Objectives

To identify the possibility of using serum miR-216a and miR-375 as biomarkers for the diagnosis and evaluation of treatment response in dogs with naturally occurring AP.

Animals

Twenty-one dogs with AP and 20 healthy dogs.

Methods

Cross-sectional study. The relative expression of serum hsa-miR-216a-5p, cfa-miR-216a, and cfa-miR-375 were analyzed using reverse transcription and real-time PCR.

Results

A significant difference in the serum expression of cfa-miR-375 was found between dogs with AP (median [interquartile range] 3.59 [1.55-24.52]-fold) and healthy d

SUBMITTER: Lee D 

PROVIDER: S-EPMC9889605 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature