Nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine.

ABSTRACT:

Introduction

Macrophages play an important role in intestinal wound healing. However, the trajectories from circulating monocytes to gut macrophages are incompletely understood.

Methods

Taking advantage of mice depleted for non-classical monocytes due to deficiency for the transcription factor Nr4a1, we addressed the relevance of non-classical monocytes for large intestinal wound healing using flow cytometry, in vivo wound healing assays and immunofluorescence.

Results

We show that wound healing in Nr4a1-deficient mice is substantially delayed and associated with reduced peri-lesional presence of macrophages with a wound healing phenotype.

Discussion

Our data suggest that non-classical monocytes are biased towards wound healing macrophages. These insight

SUBMITTER: Heidbreder K 

PROVIDER: S-EPMC9890957 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature