Establishing a simple perfusion cell culture system for light-activated liposomes.

ABSTRACT: The off-target effects of light-activated or targeted liposomes are difficult to distinguish in traditional well plate experiments. Additionally, the absence of fluid flow in traditional cell models can lead to overestimation of nanoparticle uptake. In this paper, we established a perfusion cell culture platform to study light-activated liposomes and determined the effect of flow on the liposomal cell uptake. The optimal cell culturing parameters for the A549 cells under flow conditions were determined by monitoring cell viability. To determine optimal liposome treatment times, particle uptake was measured with flow cytometry. The suitability of commercial QuasiVivo flow-chambers for near-infrared light activation was assessed with a calcein release study. The chamber material did not hind

SUBMITTER: Maki-Mikola E 

PROVIDER: S-EPMC9899206 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature