Spatiotemporal local and abscopal cell death and immune responses to histotripsy focused ultrasound tumor ablation.

ABSTRACT:

Introduction

Histotripsy is a novel focused ultrasound tumor ablation modality with potent immunostimulatory effects.

Methods

To measure the spatiotemporal kinetics of local andabscopal responses to histotripsy, C57BL/6 mice bearing bilateral flank B16 melanoma or Hepa1-6 hepatocellular carcinoma tumors were treated with unilateral sham or partial histotripsy. Treated and contralateral untreated (abscopal) tumors were analyzed using multicolor immunofluorescence, digital spatial profiling, RNA sequencing (RNASeq), and flow cytometry.

Results

Unilateral histotripsy triggered abscopal tumor growth inhibition. Within the ablation zone, early high mobility group box protein 1 (HMGB1) release and necroptosis were accompanied by immunogenic cell death transcriptional respon

SUBMITTER: Pepple AL 

PROVIDER: S-EPMC9900174 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature