A high-throughput sequencing approach identifies immunotherapeutic targets for bacterial meningitis in neonates.

ABSTRACT:

Background

Worldwide, Escherichia coli is the leading cause of neonatal Gram-negative bacterial meningitis, but full understanding of the pathogenesis of this disease is not yet achieved. Moreover, to date, no vaccine is available against bacterial neonatal meningitis.

Methods

Here, we used Transposon Sequencing of saturated banks of mutants (TnSeq) to evaluate E. coli K1 genetic fitness in murine neonatal meningitis. We identified E. coli K1 genes encoding for factors important for systemic dissemination and brain infection, and focused on products with a likely outer-membrane or extra-cellular localization, as these are potential vaccine candidates. We used in vitro and in vivo models to study the efficacy of active and passive immunization.

Results

We selected for

SUBMITTER: Pons S 

PROVIDER: S-EPMC9900374 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature