The m6A demethylase FTO promotes keloid formation by up-regulating COL1A1.

ABSTRACT:

Background

Keloid is a dermal fibrotic disease characterized by excessive proliferation of dermal fibroblasts and deposition of excessive collagen. N6-methyladenosine (m6A) plays a significant role in numerous physiological and pathological regulatory processes in the human body. Fat mass and obesity-associated protein (FTO) is one of the most essential m6A demethylases. However, whether FTO has a regulatory role in keloid development remains to be determined.

Methods

In this study, we investigated the effects of the m6A demethylase FTO on keloid formation by performing hematoxylin and eosin (H&E) staining, m6A dot blotting, transwell migration experiment, and methylated RNA immunoprecipitation quantitative polymerase chain reaction (MeRIP-qPCR) tests, as well as real-time P

SUBMITTER: Ren S 

PROVIDER: S-EPMC9906204 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature