Multiscale reconstruction of bronchus and cancer cells in human lung adenocarcinoma.

ABSTRACT:

Background

While previous studies primarily focused on the structure of the normal whole mouse lung, the whole bronchus and cytoarchitectural details of the mouse intact lung lobe have been discovered at single-cell resolution. Revealing the sophisticated lung adenocarcinoma structure at three-dimensional (3D) and single-cell level remains a fundamental and critical challenge for the pathological mechanism research of lung adenocarcinoma (LA).

Methods

Fluorescence micro-optical Sectioning Tomography (fMOST) combined with PI staining were used to obtain the 3D imaging of the human LA tissue at single-cell resolution.

Results

With a spatial resolution of 0.32 × 0.32 × 1.0 μm³, the dataset of human LA with single-cell precision consists of two channels, each o

SUBMITTER: Li X 

PROVIDER: S-EPMC9906908 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature