Distribution of Macrolide Resistant Mycoplasma genitalium in Urogenital Tract Specimens From Women Enrolled in a US Clinical Study Cohort.

ABSTRACT:

Background

This study evaluated the distribution of macrolide-resistant Mycoplasma genitalium in multiple urogenital specimens collected from women enrolled in a prospective multicenter US clinical study.

Methods

Four female urogenital specimens (vaginal swab, urine, endocervical swab, ectocervical brush/spatula) collected from each subject were tested using a transcription-mediated amplification (TMA) assay for M. genitalium. TMA-positive specimens were evaluated by reverse transcription-polymerase chain reaction and bidirectional Sanger sequencing of M. genitalium 23S rRNA to identify the presence of macrolide-resistance-mediating mutations (MRMs) at base positions 2058/2059.

Results

Of 140 women with ≥1 TMA-positive specimens, 128 (91.4%) yielded M. genitalium 23S

SUBMITTER: Getman D 

PROVIDER: S-EPMC9907502 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature