Versatile mapping-by-sequencing with Easymap v.2.

ABSTRACT: Mapping-by-sequencing combines Next Generation Sequencing (NGS) with classical genetic mapping by linkage analysis to establish gene-to-phenotype relationships. Although numerous tools have been developed to analyze NGS datasets, only a few are available for mapping-by-sequencing. One such tool is Easymap, a versatile, easy-to-use package that performs automated mapping of point mutations and large DNA insertions. Here, we describe Easymap v.2, which also maps small insertion/deletions (InDels), and includes workflows to perform QTL-seq and variant density mapping analyses. Each mapping workflow can accommodate different experimental designs, including outcrossing and backcrossing, F₂, M₂, and M₃ mapping populations, chemically induced mutation and natural

SUBMITTER: Lup SD 

PROVIDER: S-EPMC9909543 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature