A single-cell RNA labeling strategy for measuring stress response upon tissue dissociation.

ABSTRACT: Tissue dissociation, a crucial step in single-cell sample preparation, can alter the transcriptional state of a sample through the intrinsic cellular stress response. Here we demonstrate a general approach for measuring transcriptional response during sample preparation. In our method, transcripts made during dissociation are labeled for later identification upon sequencing. We found general as well as cell-type-specific dissociation response programs in zebrafish larvae, and we observed sample-to-sample variation in the dissociation response of mouse cardiomyocytes despite well-controlled experimental conditions. Finally, we showed that dissociation of the mouse hippocampus can lead to the artificial activation of microglia. In summary, our approach facilitates experimental optimization o

SUBMITTER: Neuschulz A 

PROVIDER: S-EPMC9912023 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature