Actin polymerisation and crosslinking drive left-right asymmetry in single cell and cell collectives.

ABSTRACT: Deviations from mirror symmetry in the development of bilateral organisms are common but the mechanisms of initial symmetry breaking are insufficiently understood. The actin cytoskeleton of individual cells self-organises in a chiral manner, but the molecular players involved remain essentially unidentified and the relationship between chirality of an individual cell and cell collectives is unclear. Here, we analysed self-organisation of the chiral actin cytoskeleton in individual cells on circular or elliptical patterns, and collective cell alignment in confined microcultures. Screening based on deep-learning analysis of actin patterns identified actin polymerisation regulators, depletion of which suppresses chirality (mDia1) or reverses chirality direction (profilin1 and CapZβ). The reve

SUBMITTER: Tee YH 

PROVIDER: S-EPMC9922260 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature