Cell-free Chromatin Immunoprecipitation to detect molecular pathways in Physiological and Disease States.

ABSTRACT: Patient monitoring is a cornerstone in clinical practice to define disease phenotypes and guide clinical management. Unfortunately, this is often reliant on invasive and/or less sensitive methods that do not provide deep phenotype assessments of disease state to guide treatment. This paper examined plasma cell-free DNA chromatin immunoprecipitation sequencing (cfChIP-seq) to define molecular gene sets in physiological and heart transplant patients taking immunosuppression medications. We show cfChIP-seq reliably detect gene signals that correlate with gene expression. In healthy controls and in heart transplant patients, cfChIP-seq reliably detected housekeeping genes. cfChIP-seq identified differential gene signals of the relevant immune and non-immune molecular pathways that were predomi

SUBMITTER: Jang MK 

PROVIDER: S-EPMC9928031 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature