[Andrographolide-releasing collagen scaffold enhance the ability of chondrocytes to maintain their specific phenotype under inflammatory environment in vitro].

ABSTRACT: The aim of this article is to study how andrographolide-releasing collagen scaffolds influence rabbit articular chondrocytes in maintaining their specific phenotype under inflammatory environment. Physical blending combined with vacuum freeze-drying method was utilized to prepare the andrographolide-releasing collagen scaffold. The characteristics of scaffold including its surface morphology and porosity were detected with environmental scanning electron microscope (ESEM) and a density instrument. Then, the release of andrographolide from prepared scaffolds was measured by UV-visible spectroscopy. Rabbit chondrocytes were isolated and cultured in vitro and seeded on andrographolide-releasing collagen scaffolds. Following culture with normal medium for 3 d, seeded chondrocytes were c

SUBMITTER: Xu L 

PROVIDER: S-EPMC9935195 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature