Electron Paramagnetic Resonance for the Detection of Electrochemically Generated Hydroxyl Radicals: Issues Associated with Electrochemical Oxidation of the Spin Trap.

ABSTRACT: For the detection of electrochemically produced hydroxyl radicals (HO^·) from the oxidation of water on a boron-doped diamond (BDD) electrode, electron paramagnetic resonance spectroscopy (EPR) in combination with spin trap labels is a popular technique. Here, we show that quantification of the concentration of HO^· from water oxidation via spin trap electrochemical (EC)-EPR is problematic. This is primarily due to the spin trap oxidizing at potentials less positive than water, resulting in the same spin trap-OH^· adduct as formed from the solution reaction of OH^· with the spin trap. We illustrate this through consideration of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as a spin trap for OH^·. DMPO oxidation on a BDD electrode in an ac

SUBMITTER: Braxton E 

PROVIDER: S-EPMC9936800 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature