Production of rAAV by plasmid transfection induces antiviral and inflammatory responses in suspension HEK293 cells.

ABSTRACT: Recombinant adeno-associated virus (rAAV) is a clinically proven viral vector for delivery of therapeutic genes to treat rare diseases. Improving rAAV manufacturing productivity and vector quality is necessary to meet clinical and commercial demand. These goals will require an improved understanding of the cellular response to rAAV production, which is poorly defined. We interrogated the kinetic transcriptional response of HEK293 cells to rAAV production following transient plasmid transfection, under manufacturing-relevant conditions, using RNA-seq. Time-series analyses identified a robust cellular response to transfection and rAAV production, with 1,850 transcripts differentially expressed. Gene Ontology analysis determined upregulated pathways, including inflammatory and antiviral respo

SUBMITTER: Chung CH 

PROVIDER: S-EPMC9937832 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature