Proximity labeling reveals a new in vivo network of interactors for the histone demethylase KDM5.

ABSTRACT:

Background

KDM5 family proteins are multi-domain regulators of transcription that when dysregulated contribute to cancer and intellectual disability. KDM5 proteins can regulate transcription through their histone demethylase activity in addition to demethylase-independent gene regulatory functions that remain less characterized. To expand our understanding of the mechanisms that contribute to KDM5-mediated transcription regulation, we used TurboID proximity labeling to identify KDM5-interacting proteins.

Results

Using Drosophila melanogaster, we enriched for biotinylated proteins from KDM5-TurboID-expressing adult heads using a newly generated control for DNA-adjacent background in the form of dCas9:TurboID. Mass spectrometry analyses of biotinylated proteins identified both

SUBMITTER: Yheskel M 

PROVIDER: S-EPMC9938590 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature