Endogenous DAF-16 spatiotemporal activity quantitatively predicts lifespan extension induced by dietary restriction.

ABSTRACT: In many organisms, dietary restriction (DR) leads to lifespan extension through the activation of cell protection and pro-longevity gene expression programs. In the nematode C. elegans, the DAF-16 transcription factor is a key aging regulator that governs the Insulin/IGF-1 signaling pathway and undergoes translocation from the cytoplasm to the nucleus of cells when animals are exposed to food limitation. However, how large is the influence of DR on DAF-16 activity, and its subsequent impact on lifespan has not been quantitatively determined. In this work, we assess the endogenous activity of DAF-16 under various DR regimes by coupling CRISPR/Cas9-enabled fluorescent tagging of DAF-16 with quantitative image analysis and machine learning. Our results indicate that DR regimes induce strong e

SUBMITTER: Huayta J 

PROVIDER: S-EPMC9941123 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature