Human gene regulatory evolution is driven by the divergence of regulatory element function in both cis and trans.

ABSTRACT: Gene regulatory divergence between species can result from cis-acting local changes to regulatory element DNA sequences or global trans-acting changes to the regulatory environment. Understanding how these mechanisms drive regulatory evolution has been limited by challenges in identifying trans-acting changes. We present a comprehensive approach to directly identify cis- and trans-divergent regulatory elements between human and rhesus macaque lymphoblastoid cells using ATAC-STARR-seq. In addition to thousands of cis changes, we discover an unexpected number (~10,000) of trans changes and show that cis and trans elements exhibit distinct patterns of sequence divergence and function. We further identify differentially expressed t

SUBMITTER: Hansen T 

PROVIDER: S-EPMC9949080 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature