Dataset Information

The Global Influence of Sodium on Cyanobacteria in Resuscitation from Nitrogen Starvation.

ABSTRACT: Dormancy and resuscitation are key to bacterial survival under fluctuating environmental conditions. In the absence of combined nitrogen sources, the non-diazotrophic model cyanobacterium Synechocystis sp. PCC 6803 enters into a metabolically quiescent state during a process termed chlorosis. This state enables the cells to survive until nitrogen sources reappear, whereupon the cells resuscitate in a process that follows a highly orchestrated program. This coincides with a metabolic switch into a heterotrophic-like mode where glycogen catabolism provides the cells with reductant and carbon skeletons for the anabolic reactions that serve to re-establish a photosynthetically active cell. Here we show that the entire resuscitation process requires the presence of sodium, a ubiquitous cation that has a broad impact on bacterial physiology. The requirement for sodium in resuscitating cells persists even at elevated CO₂ levels, a condition that, by contrast, relieves the requirement for sodium ions in vegetative cells. Using a multi-pronged approach, including the first metabolome analysis of Synechocystis cells resuscitating from chlorosis, we reveal the involvement of sodium at multiple levels. Not only does sodium play a role in the bioenergetics of chlorotic cells, as previously shown, but it is also involved in nitrogen compound assimilation, pH regulation, and synthesis of key metabolites.

SUBMITTER: Burkhardt M

PROVIDER: S-EPMC9952445 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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The Global Influence of Sodium on Cyanobacteria in Resuscitation from Nitrogen Starvation.

Burkhardt Markus M Rapp Johanna J Menzel Claudia C Link Hannes H Forchhammer Karl K

Biology 20230119 2

Dormancy and resuscitation are key to bacterial survival under fluctuating environmental conditions. In the absence of combined nitrogen sources, the non-diazotrophic model cyanobacterium <i>Synechocystis</i> sp. PCC 6803 enters into a metabolically quiescent state during a process termed chlorosis. This state enables the cells to survive until nitrogen sources reappear, whereupon the cells resuscitate in a process that follows a highly orchestrated program. This coincides with a metabolic switc ...[more]

PMID: 36829438

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Dataset Information

The Global Influence of Sodium on Cyanobacteria in Resuscitation from Nitrogen Starvation.

Publications

The Global Influence of Sodium on Cyanobacteria in Resuscitation from Nitrogen Starvation.

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