Nanobody-Based Sandwich Immunoassay for Pathogenic Escherichia coli F17 Strain Detection.

ABSTRACT: Rapid and specific detection of pathogenic bacteria in fecal samples is of critical importance for the diagnosis of neonatal diarrhea in veterinary clinics. Nanobodies are a promising tool for the treatment and diagnosis of infectious diseases due to their unique recognition properties. In this study, we report the design of a nanobody-based magnetofluorescent immunoassay for the sensitive detection of pathogenic Escherichia coli F17-positive strains (E. coli F17). For this, a camel was immunized with purified F17A protein from F17 fimbriae and a nanobody library was constructed by phage display. Two specific anti-F17A nanobodies (Nbs) were selected to design the bioassay. The first one (Nb1) was conjugated to magnetic beads (MBs) to form a complex capable of efficiently capt

SUBMITTER: Dhehibi A 

PROVIDER: S-EPMC9953962 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature