LINC00543 promotes colorectal cancer metastasis by driving EMT and inducing the M2 polarization of tumor associated macrophages.

ABSTRACT:

Background

The interaction between the tumor-microenvironment (TME) and the cancer cells has emerged as a key player in colorectal cancer (CRC) metastasis. A small proportion of CRC cells which undergo epithelial-mesenchymal transition (EMT) facilitate the reshaping of the TME by regulating various cellular ingredients.

Methods

Immunohistochemical analysis, RNA immunoprecipitation (RIP), RNA Antisense Purification (RAP), dual luciferase assays were conducted to investigate the biological function and regulation of LINC00543 in CRC. A series in vitro and in vivo experiments were used to clarify the role of LINC00543 in CRC metastasis.

Results

Here we found that the long non-coding RNA LINC00543, was overexpressed in colorectal cancer tissues, which correlated with adva

SUBMITTER: Zheng J 

PROVIDER: S-EPMC9960415 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature