Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression.

ABSTRACT: Synthetic biology tools for regulating gene expression have many useful biotechnology and therapeutic applications. Most tools developed for this purpose control gene expression at the level of transcription, and relatively few methods are available for regulating gene expression at the translational level. Here, we design and engineer split orthogonal aminoacyl-tRNA synthetases (o-aaRS) as unique tools to control gene translation in bacteria and mammalian cells. Using chemically induced dimerization domains, we developed split o-aaRSs that mediate gene expression by conditionally suppressing stop codons in the presence of the small molecules rapamycin and abscisic acid. By activating o-aaRSs, these molecular switches induce stop codon suppression, and in their absence stop codon suppressi

SUBMITTER: Jiang HK 

PROVIDER: S-EPMC9974479 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature