DNA methylation profiling reveals novel pathway implicated in cardiovascular diseases of diabetes.

ABSTRACT:

Objective

Epigenetics was reported to mediate the effects of environmental risk factors on disease pathogenesis. We intend to unleash the role of DNA methylation modification in the pathological process of cardiovascular diseases in diabetes.

Methods

We screened differentially methylated genes by methylated DNA immunoprecipitation chip (MeDIP-chip) among the enrolled participants. In addition, methylation-specific PCR (MSP) and gene expression validation in peripheral blood of participants were utilized to validate the DNA microarray findings.

Results

Several aberrantly methylated genes have been explored, including phospholipase C beta 1 (PLCB1), cam kinase I delta (CAMK1D), and dopamine receptor D5 (DRD5), which participated in the calcium signaling pathway. Meanwhi

SUBMITTER: Hu S 

PROVIDER: S-EPMC9975499 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature