A magnesium transporter is involved in the cesium ion resistance of the high-concentration cesium ion-resistant bacterium Microbacterium sp. TS-1.

ABSTRACT: Cesium ion (Cs⁺) resistance has been reported in bacteria but is poorly understood as reports on Cs⁺-resistant bacteria have been limited. We previously reported a novel Cs⁺/H⁺ antiporter CshA implicated in Cs⁺-resistance in Microbacterium sp. TS-1. The present study used the same screening method to isolate novel Cs⁺-sensitive mutants and their revertants from TS-1. A comparative mutation site analysis using whole-genome sequencing revealed that MTS1_03028 encodes the Mg²⁺ transporter MgtE and is a candidate Cs⁺ resistance-related gene. We performed a bioinformatic analysis of MTS1_03028 and complementation experiments on Cs⁺ resistance in the TS-1 MTS1_03028 mutants Mut

SUBMITTER: Ishida Y 

PROVIDER: S-EPMC9995610 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature