IL-10 modified mRNA monotherapy prolongs survival after composite facial allografting through the induction of mixed chimerism.

ABSTRACT: Vascularized composite allotransplantation has great potential in face transplantation by supporting functional restoration following tissue grafting. However, the need for lifelong administration of immunosuppressive drugs still limits its wide use. Modified mRNA (modRNA) technology provides an efficient and safe method to directly produce protein in vivo. Nevertheless, the use of IL-10 modRNA-based protein replacement, which exhibits anti-inflammatory properties, has not been shown to prolong composite facial allograft survival. In this study, IL-10 modRNA was demonstrated to produce functional IL-10 protein in vitro, which inhibited pro-inflammatory cytokines and in vivo formation of an anti-inflammatory environments. We found that without any immunosuppression, C57

SUBMITTER: Avina AE 

PROVIDER: S-EPMC9996371 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature