Robust and Easy-to-Use One-Pot Workflow for Label-Free Single-Cell Proteomics.

ABSTRACT: The analysis of ultralow input samples or even individual cells is essential to answering a multitude of biomedical questions, but current proteomic workflows are limited in their sensitivity and reproducibility. Here, we report a comprehensive workflow that includes improved strategies for all steps, from cell lysis to data analysis. Thanks to convenient-to-handle 1 μL sample volume and standardized 384-well plates, the workflow is easy for even novice users to implement. At the same time, it can be performed semi-automatized using CellenONE, which allows for the highest reproducibility. To achieve high throughput, ultrashort gradient lengths down to 5 min were tested using advanced μ-pillar columns. Data-dependent acquisition (DDA), wide-window acquisition (WWA), data-independent acquisi

SUBMITTER: Matzinger M 

PROVIDER: S-EPMC9996606 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature