Dataset Information

Whole Genome Metabolism - Capnocytophaga canimorsus (strain 5)

ABSTRACT:

Whole Genome Metabolism of "Capnocytophaga canimorsus (strain 5)"

This is a whole genome metabolism model of Capnocytophaga canimorsus (strain 5).

This model has been automatically generated by the SuBliMinaL Toolbox and libAnnotationSBML using information coming from from KEGG (release 66, April 2013, accessed via the resource's web services interface) and, where relevant, augmented with metabolic pathway information extracted from MetaCyc (version 17.0, March 2013).

This model has been produced by the path2models project and is currently hosted on BioModels Database and identified by: BMID000000140855 .

Other models with the same genus include BMID000000017402 BMID000000017403 BMID000000017404 BMID000000017405 BMID000000017406 BMID000000017407 BMID000000017408 BMID000000017409 BMID000000017410 BMID000000017411 BMID000000017412 BMID000000017413 BMID000000017414 BMID000000099944 BMID000000099945 BMID000000099946 BMID000000099947 BMID000000099948 BMID000000099949 BMID000000099950 BMID000000099951 BMID000000099952 BMID000000099953 BMID000000099954 BMID000000099955 BMID000000099956 BMID000000099957 BMID000000099958 BMID000000099959 BMID000000099960 BMID000000099961 BMID000000099962 BMID000000099963 BMID000000099964 BMID000000099965 BMID000000099966 BMID000000099967 BMID000000099968 BMID000000099969 BMID000000099970 BMID000000099971 BMID000000099972 BMID000000099973 BMID000000099974 BMID000000099975 BMID000000099976 BMID000000099977 BMID000000099978 BMID000000099979 BMID000000099980 BMID000000099981 BMID000000099982 BMID000000099983 BMID000000099984 BMID000000099985 BMID000000099986 BMID000000099987 BMID000000099988 BMID000000099989 BMID000000099990 BMID000000099991 BMID000000099992 BMID000000099993 BMID000000099994 BMID000000099995 BMID000000099996 BMID000000099997 BMID000000099998 BMID000000099999 BMID000000100000 BMID000000100001 BMID000000100002 BMID000000100003 BMID000000100004 BMID000000100005 BMID000000100006 BMID000000100007 BMID000000100008 BMID000000100009 BMID000000100010 BMID000000100011 BMID000000100012 BMID000000100013 BMID000000100014 BMID000000100015 BMID000000100016 BMID000000100017 BMID000000100018 BMID000000141193 .

To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

SUBMITTER: Path2Models project

PROVIDER: BMID000000140855 | biostudies-other |

SECONDARY ACCESSION(S): 24180668

REPOSITORIES: biostudies-other

ACCESS DATA

Publications

Complete genome sequence of the dog commensal and human pathogen Capnocytophaga canimorsus strain 5.

Manfredi Pablo P Pagni Marco M Cornelis Guy R GR

Journal of bacteriology 20111001 19

Capnocytophaga canimorsus is a commensal Gram-negative bacterium, originally isolated from a dog's mouth, that causes septicemia in humans. C. canimorsus has the unusual ability to feed on host cells, including phagocytes. This capacity depends on surface-exposed glycan-foraging systems. Here we present the first complete genome sequence of a C. canimorsus strain (Cc5). ...[more]

PMID: 21914877

Publication: 1/2

Similar Datasets

Project description:tRNAs are encoded by a large gene family, usually with several isogenic tRNAs interacting with the same codon. Mutations in the anticodon region of other tRNAs can overcome specific tRNA deficiencies. Phylogenetic analysis suggests that such mutations have occurred in evolution, but the driving force is unclear. We show that in yeast suppressor mutations in other tRNAs are able to overcome deficiency of the essential TRT2-encoded tRNAThrCGU at high temperature (40°C). Surprisingly, these tRNA suppressor mutations were obtained after whole-genome transformation with DNA from thermotolerant Kluyveromyces marxianus or Ogataea polymorpha strains, but from which the mutations did apparently not originate. We suggest that transient presence of donor DNA in the host facilitates proliferation at high temperature and thus increases the chances for occurrence of spontaneous mutations suppressing defective growth at high temperature. Whole-genome sequence analysis of three transformants revealed only four to five non-synonymous mutations of which one causing TRT2 anticodon stem stabilization and two anticodon mutations in non-threonyl-tRNAs, tRNALysCUU and tRNAeMetCAU, were causative. Both anticodon mutations suppressed lethality of TRT2 deletion and apparently caused the respective tRNAs to become novel substrates for threonyl-tRNA synthetase. LC-MS/MS data could not detect any significant mistranslation and RT-qPCR results contradicted induction of the unfolded protein response. We suggest that stress conditions have been a driving force in evolution for the selection of anticodon-switching mutations in tRNAs as revealed by phylogenetic analysis. Importance of the work In this work we have identified for the first time the causative elements in a eukaryotic organism introduced by applying whole-genome transformation and responsible for the selectable trait of interest, i.e. high temperature tolerance. Surprisingly, the whole-genome transformants contained just a few SNPs, which were unrelated to the sequence of the donor DNA. In each of three independent transformants, we have identified a SNP in a tRNA, either stabilizing the essential tRNAThrCGU at high temperature or switching the anticodon of tRNALysCUU or tRNAeMetCAU into CGU, which is apparently enough for in vivo recognition by threonyl-tRNA synthetase. LC-MS/MS analysis indeed indicated absence of significant mistranslation. Phylogenetic analysis showed that similar mutations have occurred throughout evolution and we suggest that stress conditions may have been a driving force for their selection. The low number of SNPs introduced by whole-genome transformation may favor its application for improvement of industrial yeast strains.

Dataset Information

Whole Genome Metabolism - Capnocytophaga canimorsus (strain 5)

Publications

Complete genome sequence of the dog commensal and human pathogen Capnocytophaga canimorsus strain 5.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets